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Emergence percentage of N. <t>triangulifer</t> subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)
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Emergence percentage of N. triangulifer subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

doi: 10.1098/rstb.2018.0013

Figure Lengend Snippet: Emergence percentage of N. triangulifer subimagos reared in two site waters amended with SO4 as a blend of CaSO4 and MgSO4. Each point represents the mean of three replicate rearing chambers each seeded with 15 larvae 1 day post egg hatch. (Online version in colour.)

Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

Techniques:

(a) 35SO4 uptake rates in larvae of the mayfly N. triangulifer as a function of SO4 concentrations (re-drawn from Scheibener et al. [30]). (b) Total body S concentrations in subimagos reared for their entire larval stage at different SO4 concentrations.

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

doi: 10.1098/rstb.2018.0013

Figure Lengend Snippet: (a) 35SO4 uptake rates in larvae of the mayfly N. triangulifer as a function of SO4 concentrations (re-drawn from Scheibener et al. [30]). (b) Total body S concentrations in subimagos reared for their entire larval stage at different SO4 concentrations.

Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

Techniques:

Relative mRNA expression of N. triangulifer larvae subjected to 24 h sulfate stress treatment. All data are normalized to control (ASW). Error bars represent standard deviations from the mean (n = 6). A Student's t-test was performed and an asterisk indicates significant difference compared to ASW control. (Online version in colour.)

Journal: Philosophical Transactions of the Royal Society B: Biological Sciences

Article Title: Are sulfate effects in the mayfly Neocloeon triangulifer driven by the cost of ion regulation?

doi: 10.1098/rstb.2018.0013

Figure Lengend Snippet: Relative mRNA expression of N. triangulifer larvae subjected to 24 h sulfate stress treatment. All data are normalized to control (ASW). Error bars represent standard deviations from the mean (n = 6). A Student's t-test was performed and an asterisk indicates significant difference compared to ASW control. (Online version in colour.)

Article Snippet: To compare the gene expression associated with sulfate stress, primers were designed based on a de novo assembly of compiled N. triangulifer cDNA sequence data (both 454 and Illumina platforms) resulting in approximately 23 000 contigs with associated bioinformatics.

Techniques: Expressing, Control